en>David Eppstein: /* See also */ Erdős–Hajnal conjecture

2014-04-28T06:51:11Z

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	~~\| Name = 15-cis~~-~~phytoene synthase~~
	~~\| EC_number = 2~~.~~5.1.32~~
	~~\| CAS_number = 50936~~-61-3
	~~\| IUBMB_EC_number = 2/5/1/32~~
	~~\| GO_code =~~
	~~\| image =~~
	~~\| width =~~
	~~\| caption =~~
	}}
	~~'''Phytoene synthase''' ({{EC number\|2~~.5.1.~~32}}~~, ~~''prephytoene-diphosphate synthase''~~, ~~''15-cis-phytoene synthase'', ''PSase'', ''geranylgeranyl-diphosphate geranylgeranyltransferase'') is~~ a ~~[[transferase]] enzyme involved in the biosynthesis of [[carotenoid]]~~s~~. It catalyzes~~ the ~~conversion of [[geranylgeranyl pyrophosphate]] to [[phytoene]]~~.~~<ref>~~[~~http~~://www.~~brenda-enzymes~~.~~org~~/~~php/result_flat.php4~~?~~ecno~~=2.5.1.~~32 Phytoene synthase]~~<~~/ref~~><~~ref~~>~~{{cite journal \| title = The stereochemistry of ''trans''-phytoene synthesis~~. ~~Some observations on lycopersene~~ as a ~~carotene precursor and~~ a ~~mechanism for~~ the ~~synthesis~~ of ~~''cis''~~- ~~and ''trans''-phytoene \|author = Gregonis, D.E~~. and ~~Rilling, H~~.~~C. \|journal = Biochemistry \|year = 1974 \|volume = 13 \|pages = 1538–1542 \|pmid = 4819767 \| doi = 10.1021/bi00704a033 \| issue = 7}}~~<~~/ref~~><~~ref~~>~~{{cite journal \| title = Expression of~~ a ~~tomato cDNA coding for phytoene synthase in ''Escherichia coli''~~, ~~phytoene formation in vivo~~ and in ~~vitro, and functional analysis of~~ the ~~various truncated gene products \|author = Misawa, N~~.~~, Truesdale, M~~.R.~~, Sandmann, G~~.~~, Fraser, P.D~~., ~~Bird~~, C.~~, Schuch, W. and Bramley, P~~.M. ~~\|journal~~ = J. ~~Biochem~~. ~~(Tokyo) \|year = 1994 \|volume = 116 \|pages = 980–985 \|pmid = 7896759 \| issue = 5}}~~<~~/ref~~> ~~This enzyme [[catalysis\|catalyses]] the following [[chemical reaction]]~~

	~~: 2 [[geranylgeranyl diphosphate]]~~ <~~math~~>~~\rightleftharpoons~~<~~/math~~> 15-~~cis~~-~~phytoene + 2 [[diphosphate]] (overall reaction)~~
	~~:(1a) 2 geranylgeranyl diphosphate~~ <~~math~~>~~\rightleftharpoons~~<~~/math~~> ~~diphosphate + [[prephytoene diphosphate]]~~
	~~:(1b) prephytoene diphosphate~~ <~~math~~>~~\rightleftharpoons~~</~~math> 15-cis-phytoene + diphosphate~~

	~~This enzyme requires Mn~~<~~sup~~>2+<~~/sup~~> ~~for activity. It belongs~~ to [~~[squalene~~/~~phytoene synthase family]] of proteins~~.

	~~== References ==~~
	~~{{reflist}}~~

	~~[[Category:EC 2~~.~~5.1~~]]


	~~{{transferase~~-~~stub}}~~

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{{Infobox enzyme
| Name = 15-cis-phytoene synthase
| EC_number = 2.5.1.32
| CAS_number = 50936-61-3
| IUBMB_EC_number = 2/5/1/32
| GO_code =
| image =
| width =
| caption =
}}
'''Phytoene synthase''' ({{EC number|2.5.1.32}}, ''prephytoene-diphosphate synthase'', ''15-cis-phytoene synthase'', ''PSase'', ''geranylgeranyl-diphosphate geranylgeranyltransferase'') is a [[transferase]] enzyme involved in the biosynthesis of [[carotenoid]]s. It catalyzes the conversion of [[geranylgeranyl pyrophosphate]] to [[phytoene]].<ref>[http://www.brenda-enzymes.org/php/result_flat.php4?ecno=2.5.1.32 Phytoene synthase]</ref><ref>{{cite journal | title = The stereochemistry of ''trans''-phytoene synthesis. Some observations on lycopersene as a carotene precursor and a mechanism for the synthesis of ''cis''- and ''trans''-phytoene |author = Gregonis, D.E. and Rilling, H.C. |journal = Biochemistry |year = 1974 |volume = 13 |pages = 1538–1542 |pmid = 4819767 | doi = 10.1021/bi00704a033 | issue = 7}}</ref><ref>{{cite journal | title = Expression of a tomato cDNA coding for phytoene synthase in ''Escherichia coli'', phytoene formation in vivo and in vitro, and functional analysis of the various truncated gene products |author = Misawa, N., Truesdale, M.R., Sandmann, G., Fraser, P.D., Bird, C., Schuch, W. and Bramley, P.M. |journal = J. Biochem. (Tokyo) |year = 1994 |volume = 116 |pages = 980–985 |pmid = 7896759 | issue = 5}}</ref> This enzyme [[catalysis|catalyses]] the following [[chemical reaction]]

: 2 [[geranylgeranyl diphosphate]] <math>\rightleftharpoons</math> 15-cis-phytoene + 2 [[diphosphate]] (overall reaction)
:(1a) 2 geranylgeranyl diphosphate <math>\rightleftharpoons</math> diphosphate + [[prephytoene diphosphate]]
:(1b) prephytoene diphosphate <math>\rightleftharpoons</math> 15-cis-phytoene + diphosphate

This enzyme requires Mn<sup>2+</sup> for activity. It belongs to [[squalene/phytoene synthase family]] of proteins.

== References ==
{{reflist}}

[[Category:EC 2.5.1]]

{{transferase-stub}}

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en>David Eppstein: /* See also */ Erdős–Hajnal conjecture

en>Helpful Pixie Bot: ISBNs (Build KE)