View source for 23S rRNA (guanine748-N1)-methyltransferase

{{Infobox enzyme
| Name = TRNA (cytidine<sup>32</sup>/uridine<sup>32</sup>-2'-O)-methyltransferase
| EC_number = 2.1.1.200
| CAS_number = 
| IUBMB_EC_number = 2/1/1/200
| GO_code = 
| image = 
| width = 
| caption =
}}
'''TRNA (cytidine<sup>32</sup>/uridine<sup>32</sup>-2'-O)-methyltransferase''' ({{EC number|2.1.1.200}}, ''YfhQ'', ''tRNA:Cm<sup>32</sup>/Um<sup>32</sup> methyltransferase'', ''TrMet(Xm<sup>32</sup>)'', ''TrmJ'') is an [[enzyme]] with system name ''S-adenosyl-L-methionine:tRNA (cytidine<sup>32</sup>/uridine<sup>32</sup>-2'-O)-methyltransferase''.<ref>{{cite journal | title = The ''yfhQ'' gene of ''Escherichia coli'' encodes a tRNA:Cm32/Um32 methyltransferase |author = Purta, E., van Vliet, F., Tkaczuk, K.L., Dunin-Horkawicz, S., Mori, H., Droogmans, L. and Bujnicki, J.M. |journal = BMC Mol. Biol. |year = 2006 |volume = 7 |pages = #23–23 |pmid = 16848900 | doi = 10.1186/1471-2199-7-23 | pmc = 1569432}}</ref> This enzyme [[catalysis|catalyses]] the following [[chemical reaction]]

:(1) [[S-adenosyl-L-methionine]] + [[cytidine]]<sup>32</sup> in tRNA <math>\rightleftharpoons</math> [[S-adenosyl-L-homocysteine]] + 2'-O-methylcytidine<sup>32</sup> in tRNA
:(2) S-adenosyl-L-methionine + [[uridine]]<sup>32</sup> in tRNA <math>\rightleftharpoons</math> S-adenosyl-L-homocysteine + 2'-O-methyluridine32 in tRNA

In ''[[Escherichia coli]]'' YfhQ is the only [[methyltransferase]] responsible for the formation of 2'-O-methylcytidine32 in tRNA.

== References ==
{{reflist}}

== External links ==
*{{MeshName|TRNA+(cytidine32/uridine32-2'-O)-methyltransferase}}

[[Category:EC 2.1.1]]