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{{enzyme
| Name = 4-chlorophenylacetate 3,4-dioxygenase
| EC_number = 1.14.12.9
| CAS_number = 105006-00-6
| IUBMB_EC_number = 1/14/12/9
| GO_code = 0018622
| image = 
| width = 
| caption = 
}}
In [[enzymology]], a '''4-chlorophenylacetate 3,4-dioxygenase''' ({{EC number|1.14.12.9}}) is an [[enzyme]] that [[catalysis|catalyzes]] the [[chemical reaction]]

:4-chlorophenylacetate + NADH + H<sup>+</sup> + O<sub>2</sub> <math>\rightleftharpoons</math> 3,4-dihydroxyphenylacetate + chloride + NAD<sup>+</sup>

The 4 [[substrate (biochemistry)|substrates]] of this enzyme are [[4-chlorophenylacetate]], [[nicotinamide adenine dinucleotide|NADH]], [[hydrogen ion|H<sup>+</sup>]], and [[oxygen|O<sub>2</sub>]], whereas its 3 [[product (chemistry)|products]] are [[3,4-dihydroxyphenylacetate]], [[chloride]], and [[nicotinamide adenine dinucleotide|NAD<sup>+</sup>]].

This enzyme belongs to the family of [[oxidoreductase]]s, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with NADH or NADPH as one donor, and incorporation of two atoms o oxygen into the other donor.  The systematic name of this enzyme class is '''4-chlorophenylacetate,NADH:oxygen oxidoreductase (3,4-hydroxylating, dechlorinating)'''. It employs one [[cofactor (biochemistry)|cofactor]], [[iron]].  

==References==
{{reflist|1}}
* {{cite journal | author = Markus A, Krekel D, Lingens F | date = 1986 | title = Purification and some properties of component A of the 4-chlorophenylacetate 3,4-dioxygenase from Pseudomonas species strain CBS | journal = J. Biol. Chem.  | volume = 261 | pages = 12883&ndash;8  | pmid = 3745216 | issue = 27 }}

{{1.14-enzyme-stub}}

[[Category:EC 1.14.12]]
[[Category:NADPH-dependent enzymes]]
[[Category:NADH-dependent enzymes]]
[[Category:Iron enzymes]]
[[Category:Enzymes of unknown structure]]