View source for Galactosylxylosylprotein 3-beta-galactosyltransferase

{{enzyme
| Name = glycoprotein 3-alpha-L-fucosyltransferase
| EC_number = 2.4.1.214
| CAS_number = 68247-53-0
| IUBMB_EC_number = 2/4/1/214
| GO_code = 0018392
| image = 
| width = 
| caption = 
}}
In [[enzymology]], a '''glycoprotein 3-alpha-L-fucosyltransferase''' ({{EC number|2.4.1.214}}) is an [[enzyme]] that [[catalysis|catalyzes]] the [[chemical reaction]]

:GDP-L-fucose + N<sub>4</sub>-{N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N- acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-beta-D- mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D- glucosaminyl}asparagine <math>\rightleftharpoons</math> GDP + N<sub>4</sub>-{N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N- acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-beta-D- mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-[alpha-L- fucosyl-(1->3)]-N-acetyl-beta-D-glucosaminyl}asparagine

The 5 [[substrate (biochemistry)|substrates]] of this enzyme are [[GDP-L-fucose]], [[N4-{N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-]], [[acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-beta-D-]], [[mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-]], and [[glucosaminyl}asparagine]], whereas its 5 [[product (chemistry)|products]] are [[guanosine diphosphate|GDP]], [[N4-{N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-]], [[acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-beta-D-]], [[mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-[alpha-L-]], and [[fucosyl-(1->3)]-N-acetyl-beta-D-glucosaminyl}asparagine]].

This enzyme belongs to the family of [[glycosyltransferase]]s, specifically the hexosyltransferases.  The systematic name of this enzyme class is '''GDP-L-fucose:glycoprotein (L-fucose to asparagine-linked N-acetylglucosamine of N4-{N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-a cetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-beta-D-man nosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glu cosaminyl}asparagine) 3-alpha-L-fucosyl-transferase'''. Other names in common use include '''GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha1,3-fucosyltransferase''', '''GDP-L-Fuc:Asn-linked GlcNAc alpha1,3-fucosyltransferase''', '''GDP-fucose:beta-N-acetylglucosamine (Fuc to''', '''(Fucalpha1->6GlcNAc)-Asn-peptide) alpha1->3-fucosyltransferase''', '''GDP-L-fucose:glycoprotein (L-fucose to asparagine-linked''', '''N-acetylglucosamine of''', '''4-N-{N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-''', '''acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-beta-D-''', '''mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-''', and '''glucosaminyl}asparagine) 3-alpha-L-fucosyl-transferase'''.  This enzyme participates in [[glycan structures - biosynthesis 1]].

==References==
{{reflist|1}}
* {{cite journal | author = Muller S, Hauser MT | year = 2001 | title = Cloning and expression of cDNAs encoding alpha1,3-fucosyltransferase homologues from Arabidopsis thaliana | journal = Biochim. Biophys. Acta.  | volume = 1527 | pages = 88&ndash;96  | pmid = 11420147 | issue = 1-2 }}
* {{cite journal | author = Fabini G, Freilinger A, Altmann F, Wilson IB | year = 2001 | title = Identification of core alpha 1,3-fucosylated glycans and cloning of the requisite fucosyltransferase cDNA from Drosophila melanogaster Potential basis of the neural anti-horseadish peroxidase epitope | journal = J. Biol. Chem.  | volume = 276 | pages = 28058&ndash;67  | pmid = 11382750 | doi = 10.1074/jbc.M100573200 | issue = 30 }}
* {{cite journal | author = Leiter H, Mucha J, Staudacher E, Grimm R, Glossl J, Altmann F | year = 1999 | title = Purification, cDNA cloning, and expression of GDP-L-Fuc:Asn-linked GlcNAc alpha1,3-fucosyltransferase from mung beans | journal = J. Biol. Chem.  | volume = 274 | pages = 21830&ndash;9  | pmid = 10419500 | doi = 10.1074/jbc.274.31.21830 | issue = 31 }}
* {{cite journal | author = van Tetering A, Schiphorst WE, van den Eijnden DH, van Die I | year = 1999 | title = Characterization of a core alpha1-->3-fucosyltransferase from the snail Lymnaea stagnalis that is involved in the synthesis of complex-type N-glycans | journal = FEBS. Lett.  | volume = 461 | pages = 311&ndash;4  | pmid = 10567717 | doi = 10.1016/S0014-5793(99)01489-1 | issue = 3 }}
* {{cite journal | author = JP, Hard K, Vliegenthart JF | year = 1991 | title = GDP-fucose: beta-N-acetylglucosamine (Fuc to (Fuc alpha 1--6GlcNAc)-Asn-peptide)alpha 1--3-fucosyltransferase activity in honeybee (Apis mellifica) venom glands. The difucosylation of asparagine-bound N-acetylglucosamine | journal = Eur. J. Biochem.  | volume = 199 | pages = 745&ndash;51  | pmid = 1868856 | doi = 10.1111/j.1432-1033.1991.tb16179.x | issue = 3 }}

[[Category:EC 2.4.1]]
[[Category:Enzymes of unknown structure]]


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