View source for Glucuronyl-galactosyl-proteoglycan 4-a-N-acetylglucosaminyltransferase

{{Infobox enzyme
| Name = Protein O-GlcNAc transferase
| EC_number = 2.4.1.255
| CAS_number = 
| IUBMB_EC_number = 2/4/1/255
| GO_code = 
| image = 
| width = 
| caption =
}}
'''Protein O-GlcNAc transferase''' ({{EC number|2.4.1.255}}, ''O-GlcNAc transferase'', ''OGTase'', ''O-linked N-acetylglucosaminyltransferase'', ''uridine diphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyltransferase'', ''protein O-linked beta-N-acetylglucosamine transferase'') is an [[enzyme]] with system name ''UDP-N-acetyl-D-glucosamine:protein-O-beta-N-acetyl-D-glucosaminyl transferase''.<ref>{{cite journal | title = Molecular characterization of nucleocytosolic <em>O</em>-GlcNAc transferases of <em>Giardia lamblia</em> and <em>Cryptosporidium parvum</em> |author = Banerjee, S., Robbins, P.W. and Samuelson, J. |journal = Glycobiology |date = 2009 |volume = 19 |pages = 331-336 |pmid = 18948359 |doi=10.1093/glycob/cwn107 |issue=4}}</ref><ref>{{cite journal | title = Structural insights into mechanism and specificity of <em>O</em>-GlcNAc transferase |author = Clarke, A.J., Hurtado-Guerrero, R., Pathak, S., Schuttelkopf, A.W., Borodkin, V., Shepherd, S.M., Ibrahim, A.F. and van Aalten, D.M. |journal = EMBO J. |date = 2008 |volume = 27 |pages = 2780-2788 |pmid = 18818698 |doi=10.1038/emboj.2008.186 |issue=20}}</ref><ref>{{cite journal | title = Structural insights into the mechanism and inhibition of eukaryotic <em>O</em>-GlcNAc hydrolysis |author = Rao, F.V., Dorfmueller, H.C., Villa, F., Allwood, M., Eggleston, I.M. and van Aalten, D.M. |journal = EMBO J. |date = 2006 |volume = 25 |pages = 1569-1578 |pmid = 16541109 |doi=10.1038/sj.emboj.7601026 |issue=7}}</ref><ref>{{cite journal | title = Glycosylation of nuclear and cytoplasmic proteins. Purification and characterization of a uridine diphospho-<em>N</em>-acetylglucosamine:polypeptide  &beta;-<em>N</em>-acetylglucosaminyltransferase |author = Haltiwanger, R.S., Blomberg, M.A. and Hart, G.W. |journal = J. Biol. Chem. |date = 1992 |volume = 267 |pages = 9005-9013 |pmid = 1533623 |issue=13}}</ref><ref>{{cite journal | title = O-Linked GlcNAc transferase is a conserved nucleocytoplasmic protein containing tetratricopeptide repeats |author = Lubas, W.A., Frank, D.W., Krause, M. and Hanover, J.A. |journal = J. Biol. Chem. |date = 1997 |volume = 272 |pages = 9316-9324 |pmid = 9083068 |doi=10.1074/jbc.272.14.9316}}</ref><ref>{{cite journal | title = Structure of human <em>O</em>-GlcNAc transferase and its complex with a peptide substrate |author = Lazarus, M.B., Nam, Y., Jiang, J., Sliz, P. and Walker, S. |journal = Nature |date = 2011 |volume = 469 |pages = 564-567 |pmid = 21240259 |doi=10.1038/nature09638 |issue=7331}}</ref> This enzyme [[catalysis|catalyses]] the following [[chemical reaction]]

: (1) [[UDP-N-acetyl-D-glucosamine]] + [protein]-L-serine <math>\rightleftharpoons</math> UDP + [protein]-3-O-(N-acetyl-D-glucosaminyl)-L-serine
: (2) UDP-N-acetyl-D-glucosamine + [protein]-L-threonine <math>\rightleftharpoons</math> UDP + [protein]-3-O-(N-acetyl-D-glucosaminyl)-L-threonine

This enzyme transfers [[GlcNAc]] onto substrate proteins.

== References ==
{{reflist}}

== External links ==
* {{MeshName|Protein+O-GlcNAc+transferase}}

[[Category:EC 2.4.1]]