View source for Lycopene epsilon-cyclase

{{Infobox enzyme
| Name = Pyrrolysine—tRNAPyl ligase
| EC_number = 6.1.1.26
| CAS_number = 
| IUBMB_EC_number = 6/1/1/26
| GO_code = 
| image = 
| width = 
| caption =
}}
'''Pyrrolysine—tRNAPyl ligase''' ({{EC number|6.1.1.26}}, ''PylS'', ''pyrrolysyl-tRNA synthetase'') is an [[enzyme]] with system name ''L-pyrrolysine:tRNAPyl ligase (AMP-forming)''.<ref>{{cite journal | title = Direct charging of tRNA(CUA) with pyrrolysine in vitro and in vivo |author = Blight, S.K., Larue, R.C., Mahapatra, A., Longstaff, D.G., Chang, E., Zhao, G., Kang, P.T., Green-Church, K.B., Chan, M.K. and Krzycki, J.A. |journal = Nature |year = 2004 |volume = 431 |pages = 333–335 |pmid = 15329732 |doi=10.1038/nature02895}}</ref><ref>{{cite journal | title = An aminoacyl-tRNA synthetase that specifically activates pyrrolysine |author = Polycarpo, C., Ambrogelly, A., B&eacute;rub&eacute;, A., Winbush, S.M., McCloskey, J.A., Crain, P.F., Wood, J.L. and S&ouml;ll, D. |journal = Proc. Natl. Acad. Sci. USA |year = 2004 |volume = 101 |pages = 12450–12454 |pmid = 15314242 |doi=10.1073/pnas.0405362101 |pmc=515082}}</ref><ref>{{cite journal | title = Molecular biology: genetic code seizes pyrrolysine |author = Schimmel, P. and Beebe, K. |journal = Nature |year = 2004 |volume = 431 |pages = 257–258 |pmid = 15372017 |doi=10.1038/431257a}}</ref> This enzyme [[catalysis|catalyses]] the following [[chemical reaction]]

: ATP + L-[[pyrrolysine]] + tRNAPyl <math>\rightleftharpoons</math> AMP + [[diphosphate]] + L-pyrrolysyl-tRNAPyl

This enzyme is specific for pyrrolysine as substrate as it cannot be replaced by [[lysine]] or any of the other natural amino acids.

== References ==
{{reflist}}

== External links ==
* {{MeshName|Pyrrolysine---tRNAPyl+ligase}}

{{DEFAULTSORT:Pyrrolysine-tRNAPyl ligase}}
[[Category:EC 6.1.1]]