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{{enzyme
| Name = tRNA-pseudouridine synthase
| EC_number = 5.4.99.12
| CAS_number = 
| IUBMB_EC_number = 5/4/99/12
| GO_code = 0016439
| image = 
| width = 
| caption = 
}}
{{lowercase}}
In [[enzymology]], a '''tRNA-pseudouridine synthase I''' ({{EC number|5.4.99.12}}) is an [[enzyme]] that [[catalysis|catalyzes]] the [[chemical reaction]]

:tRNA uridine <math>\rightleftharpoons</math> tRNA pseudouridine

Hence, this enzyme has one [[substrate (biochemistry)|substrate]], [[tRNA uridine]], and one [[product (chemistry)|product]], [[tRNA pseudouridine]].

This enzyme belongs to the family of [[isomerase]]s, specifically those intramolecular [[transferase]]s transferring other groups.  The systematic name of this enzyme class is '''tRNA-uridine uracilmutase'''. Other names in common use include '''tRNA-uridine isomerase''', '''tRNA pseudouridylate synthase I''', '''transfer ribonucleate pseudouridine synthetase''', '''pseudouridine synthase''', and '''transfer RNA pseudouridine synthetase'''.  

==Structural studies==

As of late 2007, 4 [[tertiary structure|structures]] have been solved for this class of enzymes, with [[Protein Data Bank|PDB]] accession codes {{PDB link|1VS3}}, {{PDB link|2NQP}}, {{PDB link|2NR0}}, and {{PDB link|2NRE}}.

==References==
{{reflist|1}}
* {{cite journal | author = Arena F, Ciliberto G, Ciampi S, Cortese R | date = 1978 | title = Purification of pseudouridylate synthetase I from Salmonella typhimurium | journal = Nucleic. Acids. Res.  | volume = 5 | pages = 4523&ndash;36  | pmid = 370771 | doi = 10.1093/nar/5.12.4523 | issue = 12 | pmc = 342770 }}
* {{cite journal | author = Kammen HO, Marvel CC, Hardy L, Penhoet EE | date = 1988 | title = Purification, structure, and properties of Escherichia coli tRNA pseudouridine synthase I | journal = J. Biol. Chem.  | volume = 263 | pages = 2255&ndash;63  | pmid = 3276686 | issue = 5 }}

{{isomerase-stub}}

[[Category:EC 5.4.99]]
[[Category:Enzymes of known structure]]