Aerobactin synthase: Difference between revisions

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{{enzyme
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| Name = aspartate-ammonia ligase
| EC_number = 6.3.1.1
| CAS_number = 9023-69-2
| IUBMB_EC_number = 6/3/1/1
| GO_code = 0004071
| image =
| width =
| caption =
}}
In [[enzymology]], an '''aspartate-ammonia ligase''' ({{EC number|6.3.1.1}}) is an [[enzyme]] that [[catalysis|catalyzes]] the [[chemical reaction]]
 
:ATP + L-aspartate + NH<sub>3</sub> <math>\rightleftharpoons</math> AMP + diphosphate + L-asparagine
 
The 3 [[substrate (biochemistry)|substrates]] of this enzyme are [[adenosine triphosphate|ATP]], [[L-aspartate]], and [[ammonia|NH<sub>3</sub>]], whereas its 3 [[product (chemistry)|products]] are [[adenosine monophosphate|AMP]], [[diphosphate]], and [[L-asparagine]].
 
This enzyme belongs to the family of [[ligase]]s, specifically those forming carbon-nitrogen bonds as acid-D-ammonia (or amine) ligases (amide synthases).  The systematic name of this enzyme class is '''L-aspartate:ammonia ligase (AMP-forming)'''. Other names in common use include '''asparagine synthetase''', and '''L-asparagine synthetase'''. This enzyme participates in 3 [[metabolism|metabolic pathways]]: [[alanine and aspartate metabolism]], [[cyanoamino acid metabolism]], and [[nitrogen metabolism]].
 
==Structural studies==
 
As of late 2007, two [[tertiary structure|structures]] have been solved for this class of enzymes, with [[Protein Data Bank|PDB]] accession codes {{PDB link|11AS}} and {{PDB link|12AS}}.
 
==References==
{{reflist|1}}
* {{cite journal | author = RAVEL JM, NORTON SJ, HUMPHREYS JS, SHIVE W | year = 1962 | title = Asparagine biosynthesis in Lactobacillus arabinosus and its control by asparagine through enzyme inhibition and repression | journal = J. Biol. Chem. | volume = 237 | pages = 2845&ndash;9  | pmid = 14490631 }}
* {{cite journal | author = Webster GC and Varner JE | year = 1955 | title = Aspartate metabolism and asparagine synthesis in plant systems | journal = J. Biol. Chem.  | volume = 215 | pages = 91&ndash;99 | pmid=14392145}}
 
{{DEFAULTSORT:Aspartate-ammonia ligase}}
[[Category:EC 6.3.1]]
[[Category:Enzymes of known structure]]
 
 
{{ligase-stub}}

Latest revision as of 11:39, 1 May 2014

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