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[[Image:Calibration curve.gif|thumb|right|350 px|A calibration curve plot showing [[Detection limit|limit of detection]] (LOD), [[Detection limit|limit of quantification]] (LOQ), dynamic range, and limit of linearity (LOL).]]
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In [[analytical chemistry]], a '''calibration curve''' is a general method for determining the concentration of a substance in an unknown sample by comparing the unknown to a set of standard samples of known concentration.<ref>http://terpconnect.umd.edu/~toh/models/CalibrationCurve.html</ref> A calibration curve is one approach to the problem of instrument calibration; other approaches may mix the standard into the unknown, giving an [[internal standard]].
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The calibration curve is a plot of how the instrumental response, the so-called analytical signal, changes with the concentration of the [[analyte]] (the substance to be measured). The operator prepares a series of standards across a range of concentrations near the expected concentration of analyte in the unknown. The concentrations of the standards must lie within the working range of the technique (instrumentation) they are using.<ref>http://www.fda.gov/downloads/Drugs/GuidanceComplianceRegulatoryInformation/Guidances/UCM070107.pdf</ref> Analyzing each of these standards using the chosen technique will produce a series of measurements. For most analyses a plot of instrument response vs. analyte concentration will show a linear relationship. The operator can measure the response of the unknown and, using the calibration curve, can ''interpolate'' to find the concentration of analyte.
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In more general use, a calibration curve is a [[Graph of a function|curve]] or [[Table (information)|table]] for a [[measuring instrument]] which measures some parameter indirectly, giving values for the desired quantity as a function of values of [[sensor]] output. For example, a calibration curve can be made for a particular [[pressure transducer]] to determine applied [[pressure]] from [[transducer]] output (a voltage).<ref>[http://www.astm.org/Standards/D5720.htm ASTM: Static Calibration of Electronic Transducer-Based Pressure Measurement Systems] Example of calibration curve for instrumentation.</ref> Such a curve is typically used when an instrument uses a sensor whose calibration varies from one sample to another, or changes with time or use; if sensor output is consistent the instrument would be marked directly in terms of the measured unit.
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The data - the concentrations of the analyte and the instrument response for each standard - can be fit to a straight line, using [[linear regression]] analysis.  This yields a model described by the equation ''y = mx + y<small>0</small>'', where '''y''' is the instrument response, '''m''' represents the sensitivity, and '''y<sub>0</sub>''' is a constant that describes the background.  The analyte concentration ('''x''') of unknown samples may be calculated from this equation.
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Many different variables can be used as the analytical signal.  For instance, [[chromium]] (III) might be measured using a [[chemiluminescence]] method, in an instrument that contains a [[photomultiplier tube]] (PMT) as the detector. The detector converts the light produced by the sample into a voltage, which increases with intensity of light. The amount of light measured is the analytical signal.
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Most analytical techniques use a calibration curve. There are a number of advantages to this approach.  First, the calibration curve provides a reliable way to calculate the uncertainty of the concentration calculated from the calibration curve (using the statistics of the [[least squares]] line fit to the data).<ref>The details for this procedure may be found in {{cite book | author = D. A. Skoog et al. | title = Principles of Instrumental Analysis | year = 2006}}, as well as many other textbooks.</ref>  
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Second, the calibration curve provides data on an empirical relationship.  The mechanism for the instrument's response to the analyte may be predicted or understood according to some theoretical model, but most such models have limited value for real samples. (Instrumental response is usually highly dependent on the condition of the analyte, [[solvent]]s used and impurities it may contain; it could also be affected by external factors such as pressure and temperature.)
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Many theoretical relationships, such as [[fluorescence]], require the determination of an instrumental constant anyway, by analysis of one or more reference standards; a calibration curve is a convenient extension of this approach. The calibration curve for a particular analyte in a particular (type of) sample provides the empirical relationship needed for those particular measurements.
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The chief disadvantages are (1) that the standards require a supply of the analyte material, preferably of high purity and in known concentration, and (2) that the standards and the unknown are in the same matrix. Some analytes - e.g., particular proteins - are extremely difficult to obtain pure in sufficient quantity. Other analytes are often in complex matrices, e.g., heavy metals in pond water. In this case, the matrix may interfere with or attenuate the signal of the analyte. Therefore a comparison between the standards (which contain no interfering compounds) and the unknown is not possible. The method of [[standard addition]] is a way to handle such a situation.
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As expected, the concentration of the unknown will have some error which can be calculated from the formula below.<ref>http://www.chem.utoronto.ca/coursenotes/analsci/StatsTutorial/ConcCalib.html</ref><ref>http://alpha.chem.umb.edu/chemistry/ch361/Salter%20jchem%20ed%201.pdf</ref> This formula assumes that a linear relationship is observed for all the standards. It is important to note that the error in the concentration will be minimal if the signal from the unknown lies in the middle of the signals of all the standards (the term <math>y_{unk}-\bar{y}</math> goes to zero if <math>y_{unk}=\bar{y}</math>)
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<math>
s_x=\frac{s_y}{|m|}\sqrt{\frac{1}{n}+\frac{1}{k}+\frac{(y_{unk}-\bar{y})^2}{m^2\sum{(x_i-\bar{x})^2}}}
</math>
 
*<math>s_y</math> is the standard deviation in the residuals <math>=\sqrt{\frac{\sum{(y_i-mx_i-b)}^2}{n-2}}</math>
*<math>m</math> is the slope of the line
*<math>b</math> is the y-intercept of the line
*<math>n</math> is the number standards
*<math>k</math> is the number of replicate unknowns
*<math>y_{unknown}</math> is the measurement of the unknown
*<math>\bar{y}</math> is the average measurement of the standards
*<math>x_i</math> are the concentrations of the standards
*<math>\bar{x}</math> is the average concentration of the standards
 
==Applications==
*Analysis of concentration
*Verifying the proper functioning of an analytical instrument or a [[Sensor#Chemical sensor|sensor]] device such as an [[ion selective electrode]]
*Determining the basic effects of a control treatment (such as a dose-survival curve in [[clonogenic assay]])
 
==Notes==
{{Reflist}}
 
==Bibliography==
{{refbegin}}
*{{cite book |author=Harris, Daniel Charles |title=Quantitative chemical analysis |publisher=W.H. Freeman |location=San Francisco |year=2003 |pages= |isbn=0-7167-4464-3 |oclc= |doi=}}
*{{cite book |author=Skoog, Douglas A.; Holler, F. James; Crouch, Stanley R.;  |title=Principles of Instrumental Analysis |publisher=Brooks Cole |location=Pacific Grove |year= 2007|pages= 1039 |isbn=0-495-01201-7 |oclc= |doi=}}
*{{cite journal |author=Lavagnini I, Magno F |title=A statistical overview on univariate calibration, inverse regression, and detection limits: Application to gas chromatography/mass spectrometry technique |journal=Mass spectrometry reviews |volume=26 |issue=1 |pages=1–18 |year=2007 |pmid=16788893 |doi=10.1002/mas.20100}}
{{refend}}
 
{{Analytical chemistry}}
 
[[Category:Analytical chemistry]]

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Looks like these are next. We made te leap and completely switched to organic whole wheat flour. Right now we are using flour from the hard red wheat. I don possess a mill (yet) and the only organic brand obtainable in my small town is that kind. So far we made waffles, bread, cookies and a new thing for us, TORTILLAS, with this particular flour. We have learned that the food On and in these things is what should be giving it the flavor. I can believe how much sugar is in store bought breads! We can tell the difference for sure. :)

The site is curated by Gerard Beirne an Irish poet and novelist now residing in Canada. His new novel, Charlie Tallulah, is forthcoming this Fall. His newest collection of poetry is Games of Chance:A Gamblers Manual (Oberon Press). Again and again in this his superbly reflexive fourth collection areas of the world are compared to linguistic concepts, to wordplay and languagestuff, to punctuation, metaphor and translation. Praise of Sprinklers for instance, memorably suggests how resembles / a crossword left unfinished on the stoop / while sassafras / gets looked up even as surely one of the collection standout achievements, invites your reader or dedicatee to you are this poem /moments prior to it being translated to track an irresistible but terrifying reconfiguration into something only halfunderstood.

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