4-hydroxybenzoate 1-hydroxylase: Difference between revisions

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{{enzyme
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| Name = isoflavone 2'-hydroxylase
| EC_number = 1.14.13.53
| CAS_number = 110183-49-8
| IUBMB_EC_number = 1/14/13/53
| GO_code = 0047957
| image =
| width =
| caption =
}}
In [[enzymology]], a '''4'-methoxyisoflavone 2'-hydroxylase''' ({{EC number|1.14.13.53}}) is an [[enzyme]] that [[catalysis|catalyzes]] the [[chemical reaction]]
 
:formononetin + NADPH + H<sup>+</sup> + O<sub>2</sub> <math>\rightleftharpoons</math> 2'-hydroxyformononetin + NADP<sup>+</sup> + H<sub>2</sub>O
 
The 4 [[substrate (biochemistry)|substrates]] of this enzyme are [[formononetin]], [[nicotinamide adenine dinucleotide phosphate|NADPH]], [[hydrogen ion|H<sup>+</sup>]], and [[oxygen|O<sub>2</sub>]], whereas its 3 [[product (chemistry)|products]] are [[2'-hydroxyformononetin]], [[nicotinamide adenine dinucleotide phosphate|NADP<sup>+</sup>]], and [[water|H<sub>2</sub>O]].
 
This enzyme belongs to the family of [[oxidoreductase]]s, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with NADH or NADPH as one donor, and incorporation of one atom o oxygen into the other donor.  The systematic name of this enzyme class is '''formononetin,NADPH:oxygen oxidoreductase (2'-hydroxylating)'''. Other names in common use include '''isoflavone 2'-monooxygenase (ambiguous)''', and '''isoflavone 2'-hydroxylase (ambiguous)'''. This enzyme participates in [[isoflavonoid biosynthesis]].  It employs one [[cofactor (biochemistry)|cofactor]], [[heme]]. 
 
== References ==
{{reflist|1}}
* {{cite journal | author = Hinderer W, Flentje U and Barz W | date = 1987 | title = Microsomal isoflavone 2'-hydroxylases and 3'-hydroxylases from chickpea (Cicer arietinum L) cell-suspensions induced for pterocarpan phytoalexin formation | journal = FEBS Lett. | volume = 214 | pages = 101&ndash;106 | doi = 10.1016/0014-5793(87)80021-2 }}
 
{{1.14.13-enzyme-stub}}
 
[[Category:EC 1.14.13]]
[[Category:NADPH-dependent enzymes]]
[[Category:Heme enzymes]]
[[Category:Enzymes of unknown structure]]
[[Category:Isoflavonoids metabolism]]

Latest revision as of 14:46, 10 August 2014

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