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The data contained in this document is the proprietary and unique property of StreeSine Singapore Pte. Ltd. (""SSPL"") except as in any other case indicated. The information contained herein is for informational functions solely and is not intended to exchange any professional recommendation. The views expressed are solely these of the authors.
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The information provided is for common information functions only and isn't meant to be personalised funding or financial recommendation. Motley Idiot Singapore contributor Stanley Lim would not own shares in any firms mentioned. Conduct searches on the title of the property to ensure that you can be given title to the property that's recognized within the contract and which you've got negotiated to purchase. Next yr there will be quite a no. of recent challenge launches that may sooth the market. Whether to purchase now or later may even relies on which venture / district you are looking at and the transactions happening for that space. The new property tax schedule for proprietor-occupied properties will ensure that most retirees will find yourself paying lower property taxes, he added.
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The Hillford, the first retirement resort in Singapore dropped at you by World Class Land, a subsidiary of the Aspial jewellery group. Situated in the prestige Bukit Timah space at Jalan Jurong Kechil Road, the hillford development will home a complete six blocks of residences and one bl Asian buyers gave the impression to be on the forefront, with the biggest chunk comprising investors from Singapore, whose demand has overshot that of consumers from Hong Kong. This improve has been documented by Jones Lang LaSalle, a leading guide in the actual property sector. The housing mortgage market in Singapore has evidently been rosy, these past few years. Concourse Skyline Rental & Sale Units OBTAINABLE! Reflections at Keppel Bay Rental & Sale Models OBTAINABLE! Belysa EC @ Pasir Ris
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The ampliPHOX Colorimetric Detection Technology was created as a research tool for the analysis of low-density microarrays by providing rapid, cost effective colorimetric detection with minimal user input. The combination of a simple reagent kit and a small benchtop instrument provide a powerful new detection technology for low density (<2500 spots) microarray applications and is produced by InDevR, Inc. With an investment about ten times lower than that required for a typical microarray scanner, ampliPHOX provides equivalent analytical sensitivity to fluorescence with a simple colorimetric readout.[1]
The key to ampliPHOX Colorimetric Detection Technology is a light-initiated chemical reaction that forms solid polymer spots on the microarray. Biotinylated targets are first captured onto the microarray, and subsequently labeled with a streptavidin-coupled initiator. When a proprietary solution is added and light from the ampliPHOX Reader illuminates the array, polymer grows selectively from only locations where the biotinylated targets were captured. The process occurs in just a few minutes, and can be seen with the unaided eye and subsequently imaged with the ampliPHOX Reader.
Principles of Operation
The ampliPHOX assay allows for the detection of biotin-labeled targets using light and a proprietary streptavidin-label (ampliTAG) to initiate the polymerization of a highly optimized monomer solution (ampliPHY). Transparent polymer is formed only on spots where a labeling event has occurred. The contrast of the polymer is enhanced by applying a simple stain (ampliRED) prior to imaging and analysis using the ampliVIEW software. Polymer spots formed are visible to the unaided eye. On the InDevR website, a video is available to illustrate the concept.
Biotin molecules must be incorporated into the microarray target(s) before ampliPHOX detection is performed. This can be achieved using a number of commercially available or custom options, depending on your particular application. Brief descriptions of the key steps and features of the ampliPHOX assay and ampliPHOX Reader are given below.
Labeling
The incorporation of ampliTAG onto the biotinylated microarray is the first step toward detecting the microarray capture event. Labeling the microarray with ampliTAG is performed manually and is not accomplished using the ampliPHOX Reader. The microarray labeling process consists of a five minute incubation step followed by a five minute wash.
Signal Amplification
The ampliPHY solution is applied manually to the labeled microarray and exposed to light using the instrument’s Photoactivation Bay. During photoactivation, the ampliTAG triggers polymerization of the ampliPHY. The result, after excess ampliPHY is removed, is a colorless yet visible polymer present only in regions where targets were labeled with ampliTAG. This polymer is subsequently stained with ampliRED to make the spots visible to the unaided eye and to allow for simple digital imaging and analysis of resulting signals using the ampliPHOX Reader and accompanying ampliVIEW software.
Data Analysis
After the polymer has been stained, the slide is transferred to the instrument’s Imaging Bay for analysis. First, a simple digital image of the array is captured. This image is compiled with other important information about the microarray into a data file with a “.ari” extension. Once saved, .ari files can be analyzed at any time using the Analysis Tab within the ampliVIEW software. A target is considered positive if all spots for the given target meet the following criteria:
Where s is the mean spot intensity, b is the mean background intensity (calculated from 48 pixels), and σb is the standard deviation of the background intensity.
Applications
ampliPHOX has been optimized for use with glass microarray substrates and can be utilized with both nucleic acid and protein-based systems, and in principle can be used to detect any biotin-labeled product. Research at the USDA has utilized this technology to profile Shiga toxin-producing Escherichia coli by identifying the O-antigen gene clusters and virulence genes.[2] Additionally, research at InDevR was performed to identify and type Influenza by using ampliPHOX and InDevR's Custom Microarrays in conjunction with RT-PCR. This work demonstrates that ampliPHOX Detection paired with a low-density microarray can provide a low-cost alternative to methods such as qRT-PCR for surveillance of influenza, particularly in resource-limited settings.[1]